藏鸡磷酸酪氨酸互作结构域 1（PID1）的克隆及组织表达谱研究

doi:10.11843/j.issn.0366-6964.2016.06.004

Abstract

Abstract:

In the present study，PID1 mRNA expression profile in several tissues of Tibetan chicken during different growth phases and the correlation between PID1 mRNA expression and intramuscular fat (IMF) content were investigated.PID1 gene of Tibetan chicken was cloned using RT-PCR and the structure as well as function of PID1 protein was predicted using relevant softwares.Furthermore，the expression profile of PID1 gene was investigated by qRT-PCR and the relationship between PID1 mRNA expression and IMF content in chicken muscles were analyzed.The results showed that PID1 gene was 654 bp encoding a unstable，hydrophilic，acidic protein with 217 amino acids and a PTB (phosphotyrosine binding) domain.There were 14 phosphorylation sites，4 O-glycosylation sites，1 N-glycosylation site，7 protein kinase phosphorylation sites，and 3 disulfide bonds within the PID1 protein.The predicted secondary structure of PID1 protein was composed of alpha helix (26.73%)，beta fold (20.74%) and random coil (52.53%)，belonging to one of mixed proteins in cytoplasm.qRT-PCR results showed that PID1 mRNA could be expressed in various tissues，with the highest expression level in fat (P<0.01).The temporal expression showed that the expression level of PID1 gene was the highest in breast muscle of 1 day Tibetan chicken，and was the highest in leg muscle of the 210^th day cocks (P<0.01)，while the highest in leg muscle of the 119^th day hens (P<0.01).For fat tissues，the highest expression level appeared in the 119^th day and the 154^th day cocks，and the 210^th day hens，respectively.The correlation analysis indicated that there was a significant correlation between PID1 mRNA expression and breast muscle IMF content in Tibetan chicken（P<0.05）.These results suggested that the PID1 gene might play an important role in the IMF deposition of Tibetan chicken.

CLC Number:

S831.2

NIE Xiao-qing，LIN Ya-qiu，XU Ya-ou，ZHAO Yan-ying，Lü Ming，ZUO Lu-lu，ZHANG Xiao-yu，LI Xiang. Research on Cloning and Tissue Expression Profile of Phosphotyrosine Interaction Domain Containing 1（PID1）in Tibetan Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1102-1111.

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Research on Cloning and Tissue Expression Profile of Phosphotyrosine Interaction Domain Containing 1（PID1）in Tibetan Chicken

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